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POSSIBLE APPLICATION OF METAL SENSITIVE RED FLUORESCENT PROTEINS IN ENVIRONMENTAL MONITORING
Autori: Beata Abraham, Ildiko Miklossy, Judit Petres, Laszlo Szilagyi, Maria Szabo (Palfi), Szabolcs Lanyi
Data aparitiei: January/2012
Revista: Environmental Engineering and Management JournalVol. 11Nr. 1
ISSN: 1843 - 3707
Pret: 25.00 RON    
N.A.
The metal sensitivity and selectivity of two fluorescent proteins was investigated. The studied proteins are mutant forms of the red fluorescent protein (DsRed); according to our results, both proteins showed sensitivity to the presence of copper[II] and nickel ions. The fluorescence intensity of the proteins has decreased significantly in the presence of copper ions in the micromolar range. Metal binding by these proteins is a reversible process, as addition of a chelating agent liberates the bound ions. Considering these advantageous properties, the studied proteins can be used as copper ion biosensors.


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