Abstract
Among foods that possess potent medicinal properties, Cucurbitaceae seeds stand as a promising source, since they are assumed
to have a protective effect against oxidative stress. In order to analyze the antioxidant compounds of the seeds of Citrullus
lanatus, Lagenaria siceraria, Telfaria occidentalis, Cucurbita maxima, Cucumis sativus, Cucumeropsis mannii, and Cucurbita
moschata, the dried seeds were extracted in MeOH-H2O (50 %; v/v) mixture at constant mixing rate. Qualitative phytochemical
analyses were done followed by quantitative analyses. Total phenolic and flavonoid contents were determined using the Folin-
Ciocalteu reagent and the aluminium chloride colorimetric method respectively. The alkaline precipitation method was used for
alkaloid analysis, while the tannin content was evaluated using Butanol-HCl. The gravimetric method was used to determine
saponin content. The antioxidant properties were found in all the seeds. The content in alkaloids (669 mg/100 g fw), flavonoids
(881 mg CE/100 g fw) and phenols (303.858 mg GAE/100 g fw) were significantly higher in the seeds of T. occidentalis. C.
sativus had significantly higher amounts of saponins (8.5 mg/100 g fw) and also higher amounts of tannins (145 mg LCE/100 g
fw), while C. maxima showed higher anthocyanin concentration (222 mg, c-3-gE/100 g fw). Condensed tannins (93.78 mg LCE
E/100 g fw) were most present in the seeds of C. sativus. The Friedman test allowed us to classify the seeds according to their
content in phenolics, (p <0.001): T. occidentalis >C. maxima > C. manni. These results show that our seeds have potential
protective effect against oxidative stress.