EFFECTS OF LACTIC ACID BACTERIA INOCULATION ON MOUNTAIN GRASS AND ALFALFA SILAGE FERMENTATION CHARACTERISTICS
The objective of this work was to evaluate the effects of six lactic acid bacterial strains originated from different natural habitats
of fermented food and feedstuff on pasture grass and alfalfa laboratory scale silages. The prepared mini silos were inoculated with
the following bacterial strains alone or in combination: Lactobacillus brevis LbB (2x107 cfu/mL), Enterococcus faecalis EF
(3.6x108 cfu/mL), Lactobacillus plantarum subsp. plantarum LbP (2.8x108 cfu/mL), Weissella paramesenteroides WP (3.6x107
cfu/mL), Leuconostoc lactis LL (3.5x108 cfu/mL), Lactobacillus paracasei subsp. tolerans LbPT (8.1x108 cfu/mL). The
inoculation of the grass and alfalfa laboratory silos improved the chemical composition and fermentation patterns of the silages.
Significant difference was detected (P <0.05) in crude protein content, lactic acid production and acid detergent fibre and neutral
detergent fiber content in some of the treated samples compared to the control. The best performing treatment in the case of grass
silage was the Lactobacillus brevis LbB inoculant, for alfalfa silage the most effective was the Enterococcus faecalis EF inoculant.
Also considerably good results were achived with the inoculant Lactobacillus paracasei subsp. tolerans LbPT. Our results indicate
that a bacterial consortium containing Lactobacillus brevis LbB, Enterococcus faecalis EF and Lactobacillus paracasei subsp.
tolerans LbPT could be a strategy for silage inoculant with fermentation acceleration and prevention of nutritive value loss for the
achievement of high quality silages.